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Hög känslighet detektion av Micrometastases genereras av GFP Lentivirus-sensorik Organoids odlade från en patientderiverade kolon tumör
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1Department of Coloproctological Surgery,Juntendo University Faculty of Medicine, 2Department of Molecular Pathogenesis,Juntendo University Faculty of Medicine, 3Atopy Research Center,Juntendo University Faculty of Medicine, 4Department of Oral Pathobiological Science and Surgery,Tokyo Dental College, 5Department of Gastroenterology,Juntendo University Faculty of Medicine
Capítulos
- 00:04Título
- 00:57Colorectal Cancer Cell (CRC) Organoid Artificial Extracellular Matrix Culture Installation
- 02:42CRC Organic Cell-labeling with GFP Lentiviral Particles
- 05:28GFP-labeled CRC Organoid Metastasis Generation
- 07:55Results: Representative High-resolution GFP Visualization in Cultured GFP-labeled CRC Organoids, Primary Tumors, and Micrometastases
- 08:38Conclusion
För att möjliggöra mycket känslig detektion av sprida mänskliga kolorektal cancer (CRC) celler kolonisera vävnader, visar vi häri ett protokoll för effektiv transduktion av grönt fluorescerande protein (GFP) lentiviral partiklar i PDX-derived CRC organoid celler före sin injektion i mottagarens möss, med stereo-fluorescens mikroskopisk observation.
