/
/
Adeno-associated Virus-mediated Transgene Expression in Genetically Defined Neurons of the Spinal Cord
É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
Capítulos
- 00:04Título
- 00:42Exposure, Identification, and Fixation of the Vertebrae at the Lumbar Spinal Cord Level
- 04:08Preparation of the Injection Syringe
- 05:25Intraspinal Injections
- 07:12Results: Intraspinal Injection of AAV1-eGFP/AAV1-flex-eGFP
- 08:14Conclusion
Intraspinal injection of recombinase dependent recombinant adeno-associated virus (rAAV) can be used to manipulate any genetically labelled cell type in the spinal cord. Here we describe how to transduce neurons in the dorsal horn of the lumbar spinal cord. This technique enables functional interrogation of the manipulated neuron subtype.
