/

/

腺相关病毒介导的脊髓基因神经元的转基因表达

JoVE Journal
Neurociência

É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.

JoVE Journal Neurociência

Adeno-associated Virus-mediated Transgene Expression in Genetically Defined Neurons of the Spinal Cord

Please note that all translations are automatically generated. Click here for the English version.

腺相关病毒介导的脊髓基因神经元的转基因表达

DOI:

10.3791/57382-v

•

08:41 min

•

May 12, 2018

•

Karen Haenraets*^1,2, Gioele W. Albisetti*¹, Edmund Foster, Hendrik Wildner

¹Institute of Pharmacology and Toxicology,University of Zurich, ²Institute of Pharmaceutical Sciences,Swiss Federal Institute (ETH) Zurich

Capítulos

00:04Título
00:42Exposure, Identification, and Fixation of the Vertebrae at the Lumbar Spinal Cord Level
04:08Preparation of the Injection Syringe
05:25Intraspinal Injections
07:12Results: Intraspinal Injection of AAV1-eGFP/AAV1-flex-eGFP
08:14Conclusion

Summary

Tadução automática

椎管内注射 recombinase 依赖重组腺相关病毒 (rAAV) 可以用来操纵任何基因标记的细胞类型的脊髓。在这里, 我们描述如何传感器神经元的背角的腰椎脊髓。这种技术可以对纵的神经元亚型进行功能性审问。

Tags

Adeno-associated Virus Transgene Expression Genetically Defined Neurons Spinal Cord Pain Perception Intraspinal Delivery Stereotaxic Surgery Vertebrae Identification

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

从胚胎脊髓连合神经的解剖和文化

脊髓电二：外吸电极的制作

病毒遗传背景明确的神经回路跟踪

在斑马鱼幼虫脊髓横断

对基因差异表达的RNA序列中电穿孔鸡胚脊髓分析

斑马鱼<em>原位</em>脊髓制备用于脊髓感觉和运动神经元的电生理记录

脊髓神经元从新生小鼠中分离和培养

胸腺1-gmcmp6 转基因小鼠在原代体血源皮质中的成像神经活性

拉米内切手术和脊髓窗口植入小鼠

神经干细胞向大鼠和小鼠大脑的动脉内传递：对脑缺血的应用

Read Article