/
/
Isolamento di cellule endoteliali umane da Colon normale e Carcinoma colorettale - un protocollo migliorato
This content is Free Access.
1Division of Molecular and Experimental Surgery, Department of Surgery, University Medical Center Erlangen,Friedrich-Alexander University of Erlangen-Nuremberg, 2Department of Surgery, University Medical Center Erlangen,Friedrich-Alexander University of Erlangen-Nuremberg, 3Division of Nephropathology, Department of Pathology, University Medical Center Erlangen,Friedrich-Alexander University of Erlangen-Nuremberg, 4Department of Pathology, University Medical Center Erlangen,Friedrich-Alexander University of Erlangen-Nuremberg
Capítulos
- 00:04Título
- 00:51Surgery, Preparation of Tissue, and Generation of Single Cell Suspension
- 04:17FACS Sorting, Harvesting and Characterization of Endothelial Cells
- 07:17Results: Isolation of Normal and Tumor Endothelial Cells via FACS Sorting and Study of Endothelial Cell Specific Markers
- 08:59Conclusion
Cellule endoteliali tumorali sono fattori determinanti importanti del microambiente tumorale e il decorso della malattia. Qui, un protocollo per l'isolamento di cellule endoteliali pure e praticabile da carcinoma colorettale umano e colon normale a essere impiegata nella ricerca di droga test e la patogenesi è descritto.
