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疫苗开发中佐剂细胞毒性 T 淋巴细胞生成能力的快速体内评价

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Medicina

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JoVE Journal Medicina

Rapid In Vivo Assessment of Adjuvant’s Cytotoxic T Lymphocytes Generation Capabilities for Vaccine Development

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疫苗开发中佐剂细胞毒性 T 淋巴细胞生成能力的快速体内评价

DOI:

10.3791/57401-v

•

09:03 min

•

June 19, 2018

•

Darío Lirussi, Thomas Ebensen, Kai Schulze, Elena Reinhard, Stephanie Trittel, Peggy Riese, Blair Prochnow, Carlos A. Guzmán

¹Department of Vaccinology and Applied Microbiology,Helmholtz Centre for Infection Research

Capítulos

00:05Título
01:03OT-I T Cell Staining and Adoptive Transfer
04:22Endo-Free OVA +/- Adjuvant Immunization and Lymphocyte Isolation
05:21Flow Cytometry
07:30Results: Representative Adjuvant Drive Cytotoxic T Cell Generation
08:31Conclusion

Summary

Tadução automática

我们在这里提出一个标准免疫技术 (CFSE 染色的应用), 旨在快速监测佐剂介导的细胞毒性 T 淋巴细胞 (CTL) 生成在体内.这种对 CTL 容量的快速估计有助于开发预防性疫苗, 防止细胞内病原体和治疗性癌症疫苗的发展。

Tags

Keywords: OT1 CD8 T Cells CFSE Adoptive Transfer Adjuvant Cytotoxic T Lymphocyte (CTL) Response Vaccine Development Lymph Node Spleen Magnetic Bead Isolation

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