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Isolation of Primary Human Decidual Cells from the Fetal Membranes of Term Placentae
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JoVE Journal Medicina
Isolation of Primary Human Decidual Cells from the Fetal Membranes of Term Placentae

Isolation of Primary Human Decidual Cells from the Fetal Membranes of Term Placentae

DOI:
10.3791/57443-v

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07:37 min

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April 30, 2018

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, , ,
1Department of Physiology,University of Toronto, 2Lunenfeld-Tanenbaum Research Institute,Sinai Health System, 3Department of Obstetrics and Gynecology,University of Toronto

Capítulos

  • 00:05Título
  • 00:42Collection of Decidual Tissue from Term Placental Membranes
  • 01:57Washing and Enzymatic Digestion of the Decidual Tissue
  • 03:56Generation of Single Cell Suspension
  • 05:22Results: Use of Flow Cytometry and Immunocytochemistry to Isolate the Primary Human Decidual Cells
  • 07:11Conclusion

Summary

Tadução automática

Tadução automática

This protocol demonstrates a method for the isolation of primary human decidual cells collected from the fetal membranes of term placentae which can be used for a variety of applications (i.e. immunocytochemistry, flow cytometry, etc.) aiming to study the role of different cell populations in pregnancy complications.

Tags

Primary Decidual CellsHuman ReproductionBlastocyst ImplantationLabor InitiationPregnancy-related PathologiesCell Isolation ProtocolTerm PlacentaEnzymatic DigestionCell Viability

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