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用无标签荧光显微镜评价活体、人体阻力动脉中胶原蛋白和弹性蛋白的压力依赖性 Microarchitectures

JoVE Journal
Bioengenharia

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JoVE Journal Bioengenharia

Assessing Collagen and Elastin Pressure-dependent Microarchitectures in Live, Human Resistance Arteries by Label-free Fluorescence Microscopy

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用无标签荧光显微镜评价活体、人体阻力动脉中胶原蛋白和弹性蛋白的压力依赖性 Microarchitectures

DOI:

10.3791/57451-v

•

09:58 min

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April 09, 2018

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Maria Bloksgaard, Bjarne Thorsted, Jonathan R. Brewer, Jo G. R. De Mey³

¹Department of Cardiovascular and Renal Research, Institute of Molecular Medicine,University of Southern Denmark, ²Department of Biochemistry and Molecular Biology,University of Southern Denmark, ³Department of Cardiac, Thoracic and Vascular Surgery,Odense University Hospital

Capítulos

00:04Título
01:02Imaging of the Artery Using TPEM
02:47Diameter and Wall Thickness Measurement
03:33Imaging the Collagen and Elastin Microarchitecture
05:10Image Analysis Internal Elastic Lamina Branching Angles
05:51Image Analysis – Collagen Fiber Straightness
07:46Results: Measurement of the Microstructural Changes in Adventitial Collagen and the Internal Elastic Lamina in Response to Vessel Pressure
08:48Conclusion

Summary

Tadução automática

我们描述同时进行的机械测试和 3 d 成像的动脉壁的孤立, 活着的人的抵抗动脉, 斐济和 Ilastik 图像分析, 以量化的弹性蛋白和胶原的空间组织和体积密度。我们讨论了这些数据在动脉壁力学数学模型中的应用。

Tags

Collagen Elastin Microarchitecture Resistance Arteries Label-free Fluorescence Microscopy Two-photon Microscopy Extracellular Matrix Vascular Mechanics Artery Pressurization Second Harmonic Generation Autofluorescence Fiji Image Analysis

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