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流式细胞术对小鼠原发性胰腺导管腺癌原位同种异体 (自体) 免疫分型的研究

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Immunophenotyping of Orthotopic Homograft (Syngeneic) of Murine Primary KPC Pancreatic Ductal Adenocarcinoma by Flow Cytometry

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流式细胞术对小鼠原发性胰腺导管腺癌原位同种异体 (自体) 免疫分型的研究

DOI:

10.3791/57460-v

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08:30 min

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October 09, 2018

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Xiaoyu An*^1,2, Xuesong Ouyang*¹, Hui Zhang, Tingting Li, Yu-yang Huang, Zhiyuan Li, Demin Zhou, Qi-Xang Li²

¹Crown Bioscience Inc., ²State Key Laboratory of Natural and Biomimetic Drugs,Peking University

Capítulos

00:04Título
00:33Preparation for Tumor Transplantation
01:40Surgical Operation for Orthotopic Implantation
02:58Tumor Tissue Dissociation and Single Cell Preparation
05:01Flow Data Acquisition
06:46Results: Comparison of Orthotopic and Subcutaneous Implantation of Pancreatic Ductal Adenocarcinoma
08:04Conclusion

Summary

Tadução automática

免疫分型小鼠原位 PDAC homografts 的实验程序旨在分析肿瘤免疫微环境。肿瘤通过手术原位植入。200–600毫米³的肿瘤被收获和分离, 以制备单细胞悬浮液, 然后使用不同的荧光标记抗体进行多免疫标记物的分析。

Tags

Keywords: Immunophenotyping Murine Primary KPC Pancreatic Ductal Adenocarcinoma Flow Cytometry C57 Black 6 Mice Tumor Volume Orthotopic Homograft Syngeneic Euthanasia Tumor Dissection Tumor Implantation Tumor Monitoring

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