/

/

MicroRNA 移植成人骨髓造血干细胞促进细胞工程与磁性靶向结合的协议

JoVE Journal
Bioengenharia

É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.

JoVE Journal Bioengenharia

Protocol for MicroRNA Transfer into Adult Bone Marrow-derived Hematopoietic Stem Cells to Enable Cell Engineering Combined with Magnetic Targeting

Please note that all translations are automatically generated. Click here for the English version.

MicroRNA 移植成人骨髓造血干细胞促进细胞工程与磁性靶向结合的协议

DOI:

10.3791/57474-v

•

11:37 min

•

June 18, 2018

•

Frauke Hausburg*^1,2, Paula Müller*^1,2, Natalia Voronina*¹, Gustav Steinhoff², Robert David²

¹Reference and Translation Center for Cardiac Stem Cell Therapy (RTC), Department of Cardiac Surgery,Rostock University Medical Center, ²Department Life, Light and Matter of the Interdisciplinary Faculty,Rostock University

Capítulos

00:04Título
00:53CD133 SC Isolation
06:00Characterization of Freshly Isolated CD133 SCs
07:21Preparation of Transfection Complexes
08:26CD133 SCs Transfection and Analysis
10:03Results: Successful Engineering of miR-loaded Magnetized CD133 Cells
11:02Conclusion

Summary

Tadução automática

该协议说明了一个安全和有效的程序, 以修改 CD133⁺造血干细胞。所提出的非病毒, 磁性 polyplex 公司的方法可以为优化治疗干细胞效应, 并通过磁共振成像监测所管理的细胞产品提供了依据。

Tags

Keywords: MicroRNA Transfer Adult Bone Marrow-derived Hematopoietic Stem Cells Cell Engineering Magnetic Targeting Regenerative Medicine Hematopoietic Stem Cell Modification Mesenchymal Stem Cells Bone Marrow Collection Density Gradient Centrifugation Mononuclear Cell Isolation Magnetic Selection

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

人类血管周围干细胞用于骨再生

海藻酸钠微胶囊作为一个人类胚胎干细胞（胚胎干细胞）的繁殖和分化的3D平台，以不同的谱系

在肌肉再生的老鼠模型中移植诱导多能干细胞衍生的中生代细胞样肌原体

肿瘤微环境基质与应用癌细胞侵袭组织工程

订货单细胞和单胚胎的三维约束：高内涵筛选新设备

细胞标记和超顺磁性氧化铁纳米粒子靶向

一种利用共振能量转移（FRET）的生物传感器-force议定书衡量所有核LINC复杂的机械力

3D磁干细胞聚集和生物反应器为成熟软骨再生

用原位滞后测量进行磁畴成像的优化设置和协议

使用无细胞细菌提取物、脂质体和乳液转移制备蛋白质生成合成细胞

Read Article