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Brug af Anti-phospho-girdin antistoffer til at visualisere Intestinal Tuft celler i frit svævende musen Jejunum snit frosset organmateriale
JoVE Journal
Biologia
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JoVE Journal Biologia
Use of Anti-phospho-girdin Antibodies to Visualize Intestinal Tuft Cells in Free-Floating Mouse Jejunum Cryosections

Brug af Anti-phospho-girdin antistoffer til at visualisere Intestinal Tuft celler i frit svævende musen Jejunum snit frosset organmateriale

DOI:
10.3791/57475-v

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06:26 min

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March 21, 2018

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, , , , , , ,
1Division of Perinatology, Institute for Developmental Research,Aichi Human Service Center, 2Surgery Department,Anjo Kosei Hospital, 3Department of Pathology,Nagoya University Graduate School of Medicine

Capítulos

  • 00:04Título
  • 00:40Dissection and Isolation of Jejunum
  • 02:40Immunofluorescence of Tuft Cells Using Free-floating Cryosections
  • 04:42Results: Representative Fluorescence Images of Mouse Intestinal Tuft Cells
  • 05:55Conclusion

Summary

Tadução automática

Tadução automática

Kuga et al. opdagede at fosforylering-status specifikke antistoffer mod den actin bindende protein girdin fosforyleret på tyrosin 1798 (pY1798) kan bruges til at mærke tuft celler (TCs). Denne protokol giver mulighed for robust visualisering af FKS bruger immunfluorescent farvning af frit svævende jejunum snit frosset organmateriale med pY1798 antistoffer.

Tags

Keywords: Anti-phospho-girdin AntibodiesTuft CellsMouse JejunumCryosectionsParasite InfectionGastroenterologyHistological AnalysisIntestinal ContentsFormalin FixationGelatin EmbeddingCryosectioning

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