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Levator Auris Longus Forberedelse til eksamen mammale neuromuskulære transmission spænding klemme betingelser
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Neurociência
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JoVE Journal Neurociência
Levator Auris Longus Preparation for Examination of Mammalian Neuromuscular Transmission Under Voltage Clamp Conditions

Levator Auris Longus Forberedelse til eksamen mammale neuromuskulære transmission spænding klemme betingelser

DOI:
10.3791/57482-v

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10:45 min

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May 05, 2018

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, , ,
1Department of Biological Sciences,Wright State University

Capítulos

  • 00:04Título
  • 01:18Levator Auris Longus Muscle Isolation
  • 05:22Isolation of Nerve
  • 06:50Electrophysiology Experiment
  • 08:00Identification of Neuromuscular Junction using Fluorescence
  • 08:54Results: Current-clamp and Voltage-clamp Recording of Membrane Properties
  • 09:42Conclusion

Summary

Tadução automática

Tadução automática

Den protokol, der er beskrevet i denne hvidbog bruger musen levator auris longus (LAL) muskel til at optage spontane og nerve-fremkaldte postsynaptiske potentialer (nuværende-klemme) og strøm (spænding-klemme) på den neuromuskulære junction. Brug af denne teknik kan give vigtig indsigt i mekanismer af synaptisk transmission under normal og sygdom.

Tags

Keywords: Levator Auris LongusNeuromuscular TransmissionVoltage ClampSynaptic CurrentsEnd Plate CurrentQuantal ContentProbability Of ReleaseNeurotransmissionMuscle ExcitabilitySynaptic FunctionNerve-muscle PreparationMammalianDissectionElectrophysiology

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