Quantification of Intracellular Growth Inside Macrophages is a Fast and Reliable Method for Assessing the Virulence of <em>Leishmania</em> Parasites

Amrita Sarkar; Yousuf A. Khan; Maria Fernanda Laranjeira-Silva; Norma W. Andrews; Bidyottam Mittra

doi:10.3791/57486

Journal

/

Imunologia e Infecção

/

巨噬细胞内细胞内生长的定量化是评价利什曼原虫寄生虫毒力的快速可靠方法

JoVE Journal
Imunologia e Infecção

É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.

JoVE Journal Imunologia e Infecção

Quantification of Intracellular Growth Inside Macrophages is a Fast and Reliable Method for Assessing the Virulence of Leishmania Parasites

Please note that all translations are automatically generated. Click here for the English version.

巨噬细胞内细胞内生长的定量化是评价利什曼原虫寄生虫毒力的快速可靠方法

DOI:

10.3791/57486-v

•

10:01 min

•

March 16, 2018

•

Amrita Sarkar, Yousuf A. Khan, Maria Fernanda Laranjeira-Silva, Norma W. Andrews, Bidyottam Mittra

¹Department of Cell Biology and Molecular Genetics,University of Maryland

Capítulos

00:04Título
01:08Bone Marrow-derived Macrophage (BMM) Isolation and Differentiation
04:05BMM Coverslip Plating
05:22L. amazonensis Infection and Parasite Quantification
07:21Results: Representative BMM Infection Analyses
09:06Conclusion

Summary

Tadução automática

所有致病性的利什曼原虫物种在其脊椎动物宿主的巨噬细胞内栖息和复制。在这里, 我们提出了一个协议, 以感染小鼠骨髓源性巨噬细胞的文化与利什曼原虫, 然后精确定量的细胞内生长动力学。该方法对于研究影响寄主病原体相互作用和利什曼原虫毒力的个体因素非常有用。

巨噬细胞内细胞内生长的定量化是评价利什曼原虫寄生虫毒力的快速可靠方法

•

•

•

Capítulos

Summary

Tadução automática

Tags

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

在Live主机转利什曼原虫寄生虫在体内成像

生产的灭鼠疟疾寄生虫的遗传交叉议定书“

Antileishmanial筛选对细胞内的寄生虫救援和转化实验<em>杜氏利什曼原虫</em无鞭毛体在THP1人急性单核细胞白血病细胞株

建立一个<em>在体外</em>系统来研究细胞内的行为<em>光滑念珠菌</em>在人THP-1巨噬细胞

用于适应高通量/高含量筛查的细胞内分枝杆菌定量的微观表型检测

监视分泌细菌毒力因子的组装使用站点特定的交联

ScanLag：高通量的菌落生长定量和滞后时间问题

评估隔离肺泡巨噬细胞的激光共聚焦显微镜抗真菌活性

施加诱导表达系统研究与细胞内信号细菌毒力因子的干扰

吞噬细胞内复制脓肿分枝杆菌毒力标志物的鉴定

Read Article