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Biologia
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Simple and Effective Administration and Visualization of Microparticles in the Circulatory System of Small Fishes Using Kidney Injection
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Biologia
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JoVE Journal Biologia
Simple and Effective Administration and Visualization of Microparticles in the Circulatory System of Small Fishes Using Kidney Injection

Simple and Effective Administration and Visualization of Microparticles in the Circulatory System of Small Fishes Using Kidney Injection

DOI:
10.3791/57491-v

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13:28 min

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June 17, 2018

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, , , , , ,
1Institute of Biology at Irkutsk State University, 2Institute of Biology at Karelian Research Centre of Russian Academy of Sciences, 3Baikal Research Centre, 4Institute of Materials Research and Engineering, Agency for Science, Technology, and Research (A*STAR), 5University of Oulu, Optoelectronics and Measurement Techniques Laboratory

Capítulos

  • 00:00Título
  • 02:31Fabrication of Microcapsules
  • 05:44Preparation of Optical Setup and Calibration of Microencapsulated SNARF-1
  • 08:19Preparation for Injection
  • 08:59Injection
  • 10:41In Vivo Visualization
  • 11:55Results: In Vivo Measurements of Zebrafish Plasma pH under Hypercapnia
  • 12:21Conclusion

Summary

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This article demonstrates the principles of a quick, minimally invasive injection of fluorescent microparticles into the circulatory system of small fishes and the in vivo visualization of the microparticles in fish blood.

Tags

MicroparticlesCirculatory SystemSmall FishesKidney InjectionVisualizationIntravital ImagingPH MeasurementSNARF-1Fluorescent MicrocapsulesPolyelectrolyte ShellCalcium Carbonate MicrocoresIn Vivo MonitoringBlood ParametersZebrafish

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