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一种基于昆虫蜕皮激素受体的奇异基因开关, 用于对转基因进行有鲁棒性、可逆性和可忽略的泄漏的刻意表达

JoVE Journal
Genética

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JoVE Journal Genética

An Ecdysone Receptor-based Singular Gene Switch for Deliberate Expression of Transgene with Robustness, Reversibility, and Negligible Leakiness

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一种基于昆虫蜕皮激素受体的奇异基因开关, 用于对转基因进行有鲁棒性、可逆性和可忽略的泄漏的刻意表达

DOI:

10.3791/57494-v

•

06:21 min

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May 07, 2018

•

Seoghyun Lee*¹, Minho Won*², Ran Hee Hwang, Gang Min Hur, Hyunju Ro

¹Department of Biological Sciences, College of Bioscience and Biotechnology,Chungnam National University, ²Department of Pharmacology, College of Medicine,Chungnam National University, ³Department of Nursing,Gwangju Women’s University

Capítulos

00:05Título
00:45Transient Transfection and Teb Treatment
03:00Depletion of Teb
04:01Results: Modulation of Transgene Expression of the pEUI() Singular Gene Switch using Teb
05:11Conclusion

Summary

Tadução automática

在这里, 我们提出了一种调制的转基因表达使用 pEUI (+) 奇异基因开关的米满治疗。

Tags

Keywords: Ecdysone Receptor Gene Switch Transgene Expression Tebufenozide HEK 293 Cells EGFP ANKRD13A Transfection Immunoblotting

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