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二维半变性洗涤剂琼脂糖凝胶电泳法确定淀粉样或淀粉样纤维的粒度异质性
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Bioquímica
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JoVE Journal Bioquímica
Use of Two Dimensional Semi-denaturing Detergent Agarose Gel Electrophoresis to Confirm Size Heterogeneity of Amyloid or Amyloid-like Fibers
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二维半变性洗涤剂琼脂糖凝胶电泳法确定淀粉样或淀粉样纤维的粒度异质性

DOI:
10.3791/57498-v

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10:10 min

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April 26, 2018

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,
1Department of Molecular Biology,UT Southwestern Medical Center

Capítulos

  • 00:04Título
  • 00:47Preparation of the Sample
  • 03:22Preparing and Running the Gel
  • 04:30Transfer
  • 06:07Western Blotting Detection
  • 07:45Results: Use of SDD-AGE to Confirm the Presence of Heterogenous Sizes of Amyloid-like Fibers
  • 09:24Conclusion

Summary

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在这里, 我们使用二维半变性琼脂糖凝胶电泳, 以确认存在的异质淀粉样纤维的异构大小, 并排除了大小异质性是由于淀粉样纤维在凝胶中离解的可能性运行过程。

Tags

2D Semi-denaturing Detergent Agarose Gel ElectrophoresisAmyloid FibersSize HeterogeneityProtein AggregationSDD-AGEHT-29 Colon Cancer CellsCell CultureCell LysisWhole Cell LysateSDD-AGE Buffer

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