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用光片荧光显微镜观察运动轴突导航和小鼠胚轴突树枝状的定量化

JoVE Journal
Neurociência

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JoVE Journal Neurociência

Visualization of Motor Axon Navigation and Quantification of Axon Arborization In Mouse Embryos Using Light Sheet Fluorescence Microscopy

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用光片荧光显微镜观察运动轴突导航和小鼠胚轴突树枝状的定量化

DOI:

10.3791/57546-v

•

08:56 min

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May 11, 2018

•

Ee Shan Liau*^1,2, Ya-Ping Yen*^2,3, Jun-An Chen²

¹Molecular and Cell Biology, Taiwan International Graduate Program, Academia Sinica and Graduate Institute of Life Sciences,National Defense Medical Center, ²Institute of Molecular Biology,Academia Sinica, ³Institute of Biotechnology, College of Bio-Resources and Agriculture,National Taiwan University

Capítulos

00:04Título
00:56Fixation and Whole-mount Immunostaining
02:55Sample Set-up
05:32Quantification of Axon Arborization
07:27Results: Visualization and Quantification of Motor Axon Arborization
08:22Conclusion

Summary

Tadução automática

在这里, 我们描述了一个协议, 以可视化的运动神经元投射和轴突树枝状在转基因 Hb9::GFP 小鼠胚胎. 在对运动神经元进行染色后, 我们用光片荧光显微镜对图像胚胎进行了定量分析。本协议适用于中枢神经系统的其它神经元导航过程。

Tags

Motor Axon Axon Arborization Light Sheet Fluorescence Microscopy Mouse Embryos Motor Neuron Development GFP Antibody Staining Image Analysis

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