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A Doxorubicin-induced Cardiomyopathy Model in Adult Zebrafish
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JoVE Journal Medicina
A Doxorubicin-induced Cardiomyopathy Model in Adult Zebrafish

A Doxorubicin-induced Cardiomyopathy Model in Adult Zebrafish

DOI:
10.3791/57567-v

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08:09 min

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June 07, 2018

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, , ,
1Clinical and Translational Sciences Track,Mayo Clinic Graduate School of Biomedical Sciences, 2Department of Biochemistry and Molecular Biology,Mayo Clinic, 3Division of Cardiovascular Diseases,Mayo Clinic, 4Institute of Life Science,Beijing University of Chinese Medicine

Capítulos

  • 00:04Título
  • 00:37Preparation and Quality Control of DOX Solution
  • 02:54DOX Injection
  • 05:56Results: Comparing the Two Injection Methods
  • 07:17Conclusion

Summary

Tadução automática

Tadução automática

A method to generate a doxorubicin-induced cardiomyopathy model in adult zebrafish (Danio rerio) is described here. Two alternative ways of intraperitoneal injection are presented and conditions to reduce variations among different experimental groups are discussed.

Tags

Keywords: DoxorubicinCardiomyopathyZebrafishEmbryosDechorionationQuality ControlStock SolutionDilutionHigh-throughput ScreeningAnesthetizationBody WeightDose Calculation

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