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Dorsal Root Ganglia Isolation and Primary Culture to Study Neurotransmitter Release
 

Dorsal Root Ganglia Isolation and Primary Culture to Study Neurotransmitter Release

Article DOI: 10.3791/57569-v • 08:15 min • October 6th, 2018
October 6th, 2018 •
1, 1,2,3
1Graduate Institute of Biomedical Sciences, Department of Physiology and Pharmacology, Chang Gung University, 2Healthy Aging Research Center, Chang Gung University, 3Neuroscience Research Center, Chang Gung Memorial Hospital

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Dorsal root ganglia (DRG) primary cultures are frequently used to study physiological functions or pathology-related events in sensory neurons. Here, we demonstrate the use of lumbar DRG cultures to detect the release of neurotransmitters after neuropeptide FF receptor type 2 stimulation with a selective agonist.

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Keywords: Dorsal Root Ganglia Primary Culture Neurotransmitter Release Sensory Neurons Nociception Rat Spinal Cord Lumbar DRG Collagenase Trypsin Centrifugation Cell Dissociation
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