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Imunologia e Infecção

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深部真皮注射液作为白色念珠菌皮肤感染模型的组织学分析

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Imunologia e Infecção

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JoVE Journal Imunologia e Infecção

Deep Dermal Injection As a Model of Candida albicans Skin Infection for Histological Analyses

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深部真皮注射液作为白色念珠菌皮肤感染模型的组织学分析

DOI:

10.3791/57574-v

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10:45 min

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June 13, 2018

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William Santus, Francesca Mingozzi, Marina Vai, Francesca Granucci*¹, Ivan Zanoni*^1,2

¹Department of Biotechnology and Biosciences,University of Milano-Bicocca, ²Harvard Medical School and Division of Gastroenterology,Boston Children’s Hospital

Capítulos

00:05Título
00:54C. albicans Preparation
02:29Deep Dermal Injection
03:57Sample Collection and Preparation for Histological Staining and Slides
05:09Hematoxin & Eosin Staining
06:07Periodic Acid-Schiff Staining
07:19Sample Collection and Preparation for RNA Extraction
08:30Results: Histological Analysis of C. albicans Infection in Mice
10:00Conclusion

Summary

Tadução automática

在这里, 我们描述了一个协议, 允许皮肤标本的组织学和分子分析后,白色念珠菌皮注射液。该协议维护皮肤的结构完整性, 并允许组织居民或新招募的免疫细胞的定位以及病原体的分布。

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