É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
1David H. Koch Institute for Integrative Cancer Research,Massachusetts Institute of Technology, 2Department of Respiratory Medicine, Graduate School of Medicine,The University of Tokyo, 3Hastings Center for Pulmonary Research, Division of Pulmonary, Critical Care and Sleep Medicine, Department of Medicine, Keck School of Medicine,University of Southern California, 4Department of Molecular Pathology, Graduate School of Medicine,The University of Tokyo
Capítulos
- 00:04Título
- 00:41Induction of EndMT
- 01:58Immunocytochemical Analysis
- 05:02Three-dimensional Collagen Gel Contraction Assay
- 06:09Results: Use of an In Vitro Method to Study Endothelial-mesenchymal Transition Upon TGF-β Treatment
- 07:20Conclusion
इन विट्रो में endothelial-mesenchymal संक्रमण (EndMT), जो सेलुलर संकेतन EndMT में शामिल रास्ते की जांच के लिए उपयोगी है की प्रेरण के लिए एक प्रोटोकॉल का वर्णन किया गया है । इस प्रयोगात्मक मॉडल में, EndMT एमएस-1 endothelial कोशिकाओं में TGF-β के साथ उपचार द्वारा प्रेरित है ।
