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Biologia do Desenvolvimento
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Organotypic Culture Method to Study the Development Of Embryonic Chicken Tissues
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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Organotypic Culture Method to Study the Development Of Embryonic Chicken Tissues

Organotypic Culture Method to Study the Development Of Embryonic Chicken Tissues

DOI:
10.3791/57619-v

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05:12 min

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August 25, 2018

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1Department of Biology,Mount Saint Vincent University

Capítulos

  • 00:00Título
  • 00:51Preparation
  • 01:43Embryo Preparation
  • 02:12Culture Setup
  • 03:36Results: In Vitro Culturing Supports Continued Development
  • 04:46Conclusions

Summary

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Here, we present an organotypic culturing protocol to grow embryonic chicken organs in vitro. Using this method, the development of embryonic chicken tissue can be studied, while maintaining a high degree of control over the culture environment.

Tags

Keywords: Organotypic CultureEye DevelopmentIn-vitro StudyNeural RetinaRetinal Pigmented EpitheliumAutoclavingChick SalineNutrient MediumAntibioticDissectionPetri DishWire MeshFilter Paper

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