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A Protocol to Acquire the Degenerative Tenocyte from Humans
JoVE Journal
Medicina
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JoVE Journal Medicina
A Protocol to Acquire the Degenerative Tenocyte from Humans
DOI:

09:25 min

June 09, 2018

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Capítulos

  • 00:04Título
  • 01:01Tendon Tissue Sample Extraction
  • 02:39Tenocyte Culture and Immunocytochemistry (ICC)
  • 05:10Hematoxylin and Eosin (H&E) and Immunohistochemical (IHC) Analysis
  • 07:36Results: Representative Characteristics of Degenerative Tenocytes
  • 08:59Conclusion

Summary

Tadução automática

In vitro use of degenerative tenocytes is essential when investigating the efficacy of novel treatment on tendinopathy. However, most research studies use only the animal model or a healthy tenocyte. We propose the following protocol to isolate human degenerative tenocytes during surgery.

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