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用自动光学相机进行细胞迁移的体外试验优化划痕试验
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Biologia
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JoVE Journal Biologia
Optimized Scratch Assay for In Vitro Testing of Cell Migration with an Automated Optical Camera
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用自动光学相机进行细胞迁移的体外试验优化划痕试验

DOI:
10.3791/57691-v

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05:27 min

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August 08, 2018

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,
1Department of Science and Environment,Roskilde University

Capítulos

  • 00:04Título
  • 00:23HaCaT Monolayer Generation
  • 01:50Migration Stimulation and Optical Camera Setup
  • 03:59Results: Representative Wound Healing Analyses
  • 04:54Conclusion

Summary

Tadução automática

Tadução automática

在这里, 我们描述了一个程序, 以测试的细胞迁移体外与自动光学相机显微镜。刮伤试验已被广泛使用, 在结束后的划痕是一个设定的周期。光学显微镜能够可靠、廉价地检测细胞的迁移。

Tags

Keywords: Cell MigrationScratch AssayAutomated Optical CameraHaCaT CellsTrypsinMitomycin CWound HealingCell ProliferationEndothelial Growth Factor

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