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一种简便、可再生的新分离大鼠脑微血管膜样品制备方法
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Neurociência
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JoVE Journal Neurociência
A Simple and Reproducible Method to Prepare Membrane Samples from Freshly Isolated Rat Brain Microvessels
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一种简便、可再生的新分离大鼠脑微血管膜样品制备方法

DOI:
10.3791/57698-v

•

07:13 min

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May 07, 2018

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, , ,
1Department of Pharmacology, College of Medicine,University of Arizona, Tucson

Capítulos

  • 00:04Título
  • 00:54Extraction and Processing of Brain Tissue from Sprague-Dawley Rats
  • 02:59Centrifugation
  • 04:27Results: Analysis of Freshly Isolated Rat Brain Microvessel Samples
  • 06:07Conclusion

Summary

Tadução automática

Tadução automática

本文介绍了一种分离大鼠脑微血管及制备膜样品的方法。该协议具有明显的优势, 生产丰富的微血管样品与可接受的蛋白质产量从个别动物。样品可用于大脑微血管内皮的健壮蛋白质分析。

Tags

Brain MicrovesselsBlood-brain BarrierRat BrainCerebral CortexProtease InhibitorBiochemical StudiesMembrane Isolation

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