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Interneuron 前体 Homochronic 移植早期小鼠脑的研究

JoVE Journal
Neurociência

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JoVE Journal Neurociência

Homochronic Transplantation of Interneuron Precursors into Early Postnatal Mouse Brains

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Interneuron 前体 Homochronic 移植早期小鼠脑的研究

DOI:

10.3791/57723-v

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10:08 min

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June 08, 2018

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Giulia Quattrocolo, Maria Isaac, Yajun Zhang, Timothy J. Petros

¹Kavli Institute for Systems Neuroscience and Centre for Neural Computation,Norwegian University of Science and Technology, ²Eunice Kennedy Shriver National Institute of Child Health and Human Development,National Institutes of Health

Capítulos

00:04Título
00:57Striatum, Hippocampus, and Cortex Harvest: Technique 1
03:15Striatum, Hippocampus, and Cortex Harvest: Technique 2
04:33Generation of Single Cell Suspensions
06:01P0-2 Wild-Type (WT) Pup Transplantation
08:13Results: Representative Grafted Interneuron Analyses
09:28Conclusion

Summary

Tadução automática

挑战新脑区的年轻神经元, 可以揭示环境如何造型神经元的命运和成熟的重要洞察力。本议定书描述了从特定脑区获取 interneuron 前体的程序, 并将其移植到产后幼崽的大脑中 homotopically 或 heterotopically。

Tags

Homochronic Transplantation Interneuron Precursors Postnatal Mouse Brains Developmental Neuroscience Cell Harvesting Cell Grafting Striatum Hippocampus Fluorescent Reporter Mouse Artificial Cerebrospinal Fluid Dissecting Microscope Forceps

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