Journal
/
Medicina
/
A Flow Cytometry-based Assay for Measuring Mitochondrial Membrane Potential in Cardiac Myocytes After Hypoxia/Reoxygenation
JoVE Journal
Medicina
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Medicina
A Flow Cytometry-based Assay for Measuring Mitochondrial Membrane Potential in Cardiac Myocytes After Hypoxia/Reoxygenation

A Flow Cytometry-based Assay for Measuring Mitochondrial Membrane Potential in Cardiac Myocytes After Hypoxia/Reoxygenation

DOI:
10.3791/57725-v

•

07:14 min

•

July 13, 2018

•

, , ,
1State Key Laboratory of Cardiovascular Disease, Department of Cardiology, Fuwai Hospital, National Center for Cardiovascular Diseases,Chinese Academy of Medical Sciences and Peking Union Medical College, 2State Key Laboratory of Cardiovascular Disease, Fuwai Hospital, National Center for Cardiovascular Diseases,Chinese Academy of Medical Sciences and Peking Union Medical College

Capítulos

  • 00:04Título
  • 00:42Establishment of Hypoxia/Reoxygenation Model
  • 01:57Preparation of HCMs for Flow Cytometry
  • 03:49Flow Cytometer Setup
  • 04:43Flow Cytometry Measurement and Data Analysis
  • 05:40Results: Validation of the Establishment of Hypoxia/Reoxygenation (H/R) Model by Flow Cytometry
  • 06:34Conclusion

Summary

Tadução automática

Tadução automática

Here, we present a protocol that uses JC-1 dye to assess the mitochondrial membrane potential of cells after being exposed to hypoxia/reoxygenation with or without a protective agent.

Tags

Keywords: Flow CytometryMitochondrial Membrane PotentialCardiac MyocytesHypoxiaReoxygenationJC-1Cell CultureCentrifugationTrypsinizationPhosphate Buffer SalineDMEM

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

check_url/pt/57725?article_type=v

Manual Muscle Testing: A Method of Measuring Extremity Muscle Strength Applied to Critically Ill Patients
check_url/pt/57725?article_type=v

Measuring Cardiac Autonomic Nervous System (ANS) Activity in Children
check_url/pt/57725?article_type=v

Collecting Saliva and Measuring Salivary Cortisol and Alpha-amylase in Frail Community Residing Older Adults via Family Caregivers
check_url/pt/57725?article_type=v

Imaging- and Flow Cytometry-based Analysis of Cell Position and the Cell Cycle in 3D Melanoma Spheroids
check_url/pt/57725?article_type=v

Phosphorus-31 Magnetic Resonance Spectroscopy: A Tool for Measuring In Vivo Mitochondrial Oxidative Phosphorylation Capacity in Human Skeletal Muscle
check_url/pt/57725?article_type=v

Isolation of Endothelial Progenitor Cells from Healthy Volunteers and Their Migratory Potential Influenced by Serum Samples After Cardiac Surgery
check_url/pt/57725?article_type=v

Standardized Measurement of Nasal Membrane Transepithelial Potential Difference (NPD)
check_url/pt/57725?article_type=v

Implantation of hiPSC-derived Cardiac-muscle Patches after Myocardial Injury in a Guinea Pig Model
check_url/pt/57725?article_type=v

Isolation of Atrial Myocytes from Adult Mice
check_url/pt/57725?article_type=v

Hemocompatibility Testing of Blood-Contacting Implants in a Flow Loop Model Mimicking Human Blood Flow

Read Article