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Retinal Cryo-sections, Whole-Mounts, and Hypotonic Isolated Vasculature Preparations for Immunohistochemical Visualization of Microvascular Pericytes
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Biologia
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JoVE Journal Biologia
Retinal Cryo-sections, Whole-Mounts, and Hypotonic Isolated Vasculature Preparations for Immunohistochemical Visualization of Microvascular Pericytes

Retinal Cryo-sections, Whole-Mounts, and Hypotonic Isolated Vasculature Preparations for Immunohistochemical Visualization of Microvascular Pericytes

DOI:
10.3791/57733-v

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10:46 min

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October 07, 2018

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, ,
1Department of Clinical Experimental Research,Glostrup Research Institute, 2Department of Clinical Sciences, Division of Experimental Vascular Research,Lund University

Capítulos

  • 00:04Título
  • 00:41Cryosection Rat Retinal Tissue Preparation and Immunostaining
  • 03:23Whole Mount Rat Retinal Tissue Preparation and Immunostaining
  • 05:08Hypotonic Isolation of Ray Retinal Tissue Preparation and Immunostaining
  • 08:27Results: Representative Immunohistochemistry Images
  • 10:18Conclusion

Summary

Tadução automática

Tadução automática

We demonstrate three different tissue preparation techniques for immunohistochemical visualization of rat retinal microvascular pericytes, i.e., cryo-sections, whole-mounts, and hypotonic isolation of the vascular network.

Tags

Keywords: Retinal Cryo-sectionsWhole-mountsIsolated VasculatureImmunohistochemistryPericytesEnucleationFormaldehyde FixationCryoprotectionCryosectioningAntibody StainingFluorescent LabelingMicroscopy

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