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视网膜低温切片、全支架和低渗隔离血管毛细血管微血管组织免疫组化的制备
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Biologia
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JoVE Journal Biologia
Retinal Cryo-sections, Whole-Mounts, and Hypotonic Isolated Vasculature Preparations for Immunohistochemical Visualization of Microvascular Pericytes
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视网膜低温切片、全支架和低渗隔离血管毛细血管微血管组织免疫组化的制备

DOI:
10.3791/57733-v

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10:46 min

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October 07, 2018

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, ,
1Department of Clinical Experimental Research,Glostrup Research Institute, 2Department of Clinical Sciences, Division of Experimental Vascular Research,Lund University

Capítulos

  • 00:04Título
  • 00:41Cryosection Rat Retinal Tissue Preparation and Immunostaining
  • 03:23Whole Mount Rat Retinal Tissue Preparation and Immunostaining
  • 05:08Hypotonic Isolation of Ray Retinal Tissue Preparation and Immunostaining
  • 08:27Results: Representative Immunohistochemistry Images
  • 10:18Conclusion

Summary

Tadução automática

Tadução automática

我们展示了三种不同的组织制备技术的免疫组化显示大鼠视网膜微血管毛细血管,即低温切片, 全支架, 和低渗的隔离血管网络。

Tags

Retinal Cryo-sectionsWhole-mountsIsolated VasculatureImmunohistochemistryPericytesEnucleationFormaldehyde FixationCryoprotectionCryosectioningAntibody StainingFluorescent LabelingMicroscopy

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