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Bronchoalveolar Lavage Exosomes in Lipopolysaccharide-induced Septic Lung Injury
 

Bronchoalveolar Lavage Exosomes in Lipopolysaccharide-induced Septic Lung Injury

Article DOI: 10.3791/57737-v • 08:27 min • May 21st, 2018
May 21st, 2018 •
1,2, 1, 1,2
1Department of Veterans Affairs, Atlanta VAMC, 2Division of Pulmonary and Critical Care Medicine, Department of Medicine, Emory University

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Resumo
Automatic Translation

Mice exposed to intraperitoneal LPS secrete exosomes in Broncho-alveolar lavage (BAL) fluid that are packaged with miRNAs. Using a co-culture system, we show that exosomes released in the BAL fluid disrupt expression of tight junction proteins in bronchial epithelial cells and increase expression of pro-inflammatory cytokines that accentuate lung injury.

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Keywords: Bronchoalveolar Lavage Exosomes Lipopolysaccharide Septic Lung Injury Acute Lung Injury Acute Respiratory Distress Syndrome Alveolar Microenvironment Exosome Isolation Centrifugation Ultracentrifugation Syringe Filtration Exosome Characterization
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