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激光捕获显微切割分离新鲜冷冻人体肠神经节的优化
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Neurociência
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JoVE Journal Neurociência
Optimization of Laser-Capture Microdissection for the Isolation of Enteric Ganglia from Fresh-Frozen Human Tissue
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激光捕获显微切割分离新鲜冷冻人体肠神经节的优化

DOI:
10.3791/57762-v

•

08:28 min

•

June 14, 2018

•

, ,
1Vanderbilt University Medical Center

Capítulos

  • 00:04Título
  • 00:29Preparation of Flash-Frozen Human Intestinal Tissue
  • 02:58Cryosectioning and Staining
  • 05:01Laser Capture Microdissection (LCM)
  • 07:05Results: Sequencing Data
  • 07:53Conclusion

Summary

Tadução automática

Tadução automática

该协议的目的是获得高完整性的 RNA 样本从无固定的, 新鲜切除的人肠道组织, 利用激光捕获显微切割 (LCM) 分离。该协议包括制备人体肠道组织、cryosectioning、乙醇染色和脱水、LCM 和 RNA 提取的闪光冷冻样品。

Tags

Laser-capture MicrodissectionEnteric GangliaRNA ExtractionMyenteric PlexusTaeniae ColiDry Ice2-methylbutaneCryostatTFM

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