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调频染料循环在突触: 高钾偏极化, 电和 Channelrhodopsin 刺激的比较
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Neurociência
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JoVE Journal Neurociência
FM Dye Cycling at the Synapse: Comparing High Potassium Depolarization, Electrical and Channelrhodopsin Stimulation
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调频染料循环在突触: 高钾偏极化, 电和 Channelrhodopsin 刺激的比较

DOI:
10.3791/57765-v

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08:31 min

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May 24, 2018

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1Department of Biological Sciences,Vanderbilt University, 2Departments of Biological Sciences, Pharmacology, Cell and Developmental Biology, Kennedy Center for Research on Human Development,Vanderbilt University and Medical Center

Capítulos

  • 00:04Título
  • 00:42Larval Glue Dissection
  • 02:36Electrical Stimulation: FM Dye Loading and Unloading
  • 04:08Channelrhodopsin Stimulation: FM Dye Loading and Unloading
  • 05:21Fluorescence Quantification
  • 06:12Results: Synaptic Ultrastructure with FM Dye Photoconversion to Mark Vesicles
  • 07:22Conclusion

Summary

Tadução automática

Tadução automática

突触囊泡 (SV) 循环是神经元突触中细胞间通讯的核心机制。调频染料的摄取和释放是定量测定 SV 内胞吐作用的主要手段。在这里, 我们比较所有的刺激方法, 以驱动 FM1-43 循环在果蝇神经肌肉连接 (NMJ) 模型突触。

Tags

FM DyeSynaptic Vesicle CyclingNeuromuscular JunctionDrosophila LarvaeHigh Potassium DepolarizationElectrical StimulationOptogeneticsAnti-HRP AntibodyElastomer-coated CoverslipDissectionGluingMotor NerveCalcium-free SalineSuction PipetteMicroelectrode PullerMicro ForgeMicromanipulator

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