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用 Widefield 荧光显微镜研究软硅基美国匹茨堡的刚度测量
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JoVE Journal Bioengenharia
Stiffness Measurement of Soft Silicone Substrates for Mechanobiology Studies Using a Widefield Fluorescence Microscope
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用 Widefield 荧光显微镜研究软硅基美国匹茨堡的刚度测量

DOI:
10.3791/57797-v

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07:02 min

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July 03, 2018

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, , , , ,
1Department of Mechanical & Aerospace Engineering,Old Dominion University, 2Department of Biological Sciences,Old Dominion University

Capítulos

  • 00:04Título
  • 00:42Soft Silicone Substrate Fabrication
  • 01:40Fluorescent Microbead-Soft Silicone Coupling
  • 03:34Measurement of Silicone Stiffness with Sphere Indentation
  • 04:39Silicone Stiffness Calculation (Young s Modulus)
  • 05:51Results: Representative Microbead Images and Stiffness Values
  • 06:42Conclusion

Summary

Tadução automática

Tadução automática

kilopascal 范围内的刚性基底对于研究细胞对生理相关的微环境刚度的反应是有益的。使用一个 widefield 荧光显微镜, 年轻的软硅胶凝胶的弹性模量可以确定使用一个合适的球压痕。

Tags

Stiffness MeasurementSoft Silicone SubstratesMechanobiologyWidefield Fluorescence MicroscopeYoung’s ModulusPolyacrylamide GelSelf-stretching ExperimentsSoft Silicone ElastomerAir BubblesDeep UV TreatmentEDCSulfo-NHSFluoro-4 Conjugated Carboxylate-modified Micro-beadsRat Tail Collagen 1

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