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在原位生物过程单细胞形态实时测定的显微镜
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Bioengenharia
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JoVE Journal Bioengenharia
In Situ Microscopy for Real-time Determination of Single-cell Morphology in Bioprocesses
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在原位生物过程单细胞形态实时测定的显微镜

DOI:
10.3791/57823-v

•

07:26 min

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December 05, 2019

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, , , ,
1Chair of Bioprocess Engineering, Department of Biotechnology,Technische Universität Berlin, 2SOPAT GmbH

Capítulos

  • 00:05Título
  • 01:09Overview of Probe
  • 01:40Off-line Measurement
  • 03:43Particle Identification
  • 05:11On-Line Measurement
  • 06:03Results: Cumulative Single-cell Size Distribution of S. Cerevisiae
  • 06:39Conclusion

Summary

Tadução automática

Tadução automática

研制了一种光学原位显微镜装置，用于直接监测细胞悬浮液中单个细胞的大小。通过将光光学可消毒探头与自动图像分析相结合，进行实时测量。形态变化表现为依赖生长状态和栽培条件。

Tags

Keywords: In Situ MicroscopyReal-timeSingle-cell MorphologyBioprocessesAutomated Image RecognitionCell ConcentrationFilamentous FungiYeastProbeCCD CameraMeasurement GapFocusCulture BrothPopulation HeterogeneityProcess YieldsProduct QualityContaminationCellular StructuresShapeCell Agglomeration

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