Journal
/
Genética
/
A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations
JoVE Journal
Genética
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Genética
A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

DOI:
10.3791/57831-v

•

09:34 min

•

October 25, 2018

•

, , ,
1Division of Molecular Hematology, Lund Stem Cell Center,Lund University

Capítulos

  • 00:04Título
  • 00:50Lysis Plate Preparation and Cell Sorting
  • 03:35Reverse Transcription and Specific Target Amplification
  • 04:51Preparation of Sample and Assay Plates
  • 07:41Results: Dynamic Characterization of Cell Populations
  • 08:43Conclusion

Summary

Tadução automática

Tadução automática

Bulk gene expression measurements cloud individual cell differences in heterogeneous cell populations. Here, we describe a protocol for how single-cell gene expression analysis and index sorting by Florescence Activated Cell Sorting (FACS) can be combined to delineate heterogeneity and immunophenotypically characterize molecularly distinct cell populations.

Tags

Keywords: Single-cell AnalysisStem Cell HeterogeneityCancer HeterogeneityImmunophenotypic ProfilingGene Expression AnalysisLineage PotentialFACS SortingSingle-cell PCRCombinatorial Approach

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

check_url/pt/57831?article_type=v

Single-cell Gene Expression Using Multiplex RT-qPCR to Characterize Heterogeneity of Rare Lymphoid Populations
check_url/pt/57831?article_type=v

Detection of Copy Number Alterations Using Single Cell Sequencing
check_url/pt/57831?article_type=v

Highly Efficient Gene Disruption of Murine and Human Hematopoietic Progenitor Cells by CRISPR/Cas9
check_url/pt/57831?article_type=v

Canalostomy As a Surgical Approach to Local Drug Delivery into the Inner Ears of Adult and Neonatal Mice
check_url/pt/57831?article_type=v

The Replica Set Method: A High-throughput Approach to Quantitatively Measure Caenorhabditis elegans Lifespan
check_url/pt/57831?article_type=v

Isolation, Characterization and MicroRNA-based Genetic Modification of Human Dental Follicle Stem Cells
check_url/pt/57831?article_type=v

Isolating, Sequencing and Analyzing Extracellular MicroRNAs from Human Mesenchymal Stem Cells
check_url/pt/57831?article_type=v

Preparation and Gene Modification of Nonhuman Primate Hematopoietic Stem and Progenitor Cells
check_url/pt/57831?article_type=v

Single-cell RNA Sequencing and Analysis of Human Pancreatic Islets
check_url/pt/57831?article_type=v

Generation of Defined Genomic Modifications Using CRISPR-CAS9 in Human Pluripotent Stem Cells

Read Article