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Preparation of Drosophila Larval Samples for Gas Chromatography-Mass Spectrometry (GC-MS)-based Metabolomics
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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Preparation of Drosophila Larval Samples for Gas Chromatography-Mass Spectrometry (GC-MS)-based Metabolomics

Preparation of Drosophila Larval Samples for Gas Chromatography-Mass Spectrometry (GC-MS)-based Metabolomics

DOI:
10.3791/57847-v

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07:21 min

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June 06, 2018

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,
1Department of Biology,Indiana University

Capítulos

  • 00:05Título
  • 00:37Larval Sample Collection
  • 01:28Sample Transfer to Bead Tubes
  • 02:23Sample Extraction
  • 03:52Chemical Derivatization
  • 06:20Results: Gas Chromatography-Mass Spectrometry Analyses Reveal Significant Differences Between Lactate Dehydrogenase Mutants and Wild-type Controls
  • 07:02Conclusion

Summary

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This protocol describes how to prepare Drosophila larvae for GC-MS-based metabolomic analysis.

Tags

DrosophilaLarval SamplesGas Chromatography-mass Spectrometry (GC-MS)MetabolomicsL2-hydroxyglutarateSodium ChlorideCentrifugationLiquid NitrogenBead TubeMethanolSuccinic-D4 AcidBead Mill Homogenizer

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