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高频刺激激活小鼠齿状回的侵袭方法
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Neurociência
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JoVE Journal Neurociência
An Invasive Method for the Activation of the Mouse Dentate Gyrus by High-frequency Stimulation
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高频刺激激活小鼠齿状回的侵袭方法

DOI:
10.3791/57857-v

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12:26 min

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June 02, 2018

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,
1Department of Neurobiology,Beijing Institute of Basic Medical Sciences, 2Key Laboratory of Neuroregeneration, Co-innovation Center of Neuroregeneration,Nantong University

Capítulos

  • 00:04Título
  • 01:06Electrode Implantation
  • 05:24High-Frequency Stimulation Delivery
  • 06:56Immunofluorescence Staining and Bromodeoxyuridine Labeling
  • 10:05Results: Distinct activation of neurogenesis in the DG in response to HFS in DG and PP
  • 11:46Conclusion

Summary

Tadução automática

Tadução automática

该协议说明如何在小鼠中建立可靠的痉挛方法。整个海马齿状回的神经元由痉挛直接和间接的在体内电刺激。神经活动和分子信号由c-fos和 Notch1 免疫荧光染色分别检查;bromodeoxyuridine 标记法对神经发生进行量化。

Tags

High-frequency StimulationNeuronal ActivityNeurogenesisHippocampusDeep Brain StimulationMouse Dentate GyrusStereotactic SurgeryElectrode ImplantationBrain Disorders

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