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Where You Cut Matters: A Dissection and Analysis Guide for the Spatial Orientation of the Mouse Retina from Ocular Landmarks
JoVE Journal
Neurociência
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JoVE Journal Neurociência
Where You Cut Matters: A Dissection and Analysis Guide for the Spatial Orientation of the Mouse Retina from Ocular Landmarks

Where You Cut Matters: A Dissection and Analysis Guide for the Spatial Orientation of the Mouse Retina from Ocular Landmarks

DOI:
10.3791/57861-v

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08:42 min

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August 04, 2018

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, , , ,
1Department of Biology,The University of Akron, 2Department of Cell and Developmental Biology,University of Colorado Denver

Capítulos

  • 00:04Título
  • 00:37Using the Superior Rectus Muscle Landmark to Identify Retinal Orientation
  • 02:36Using the Choroid Fissure Landmark to Identify Retinal Orientation
  • 04:06Labeling the S-opsin Gradient in the Mouse Retina
  • 07:02Results: Using the S-opsin Gradient to Identify All Four Poles of the Retina
  • 08:09Conclusion

Summary

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Tadução automática

This protocol provides a comprehensive dissection and analysis guide for the use of deep ocular landmarks, s-opsin immunohistochemistry, Retistruct, and custom code to accurately and reliably orient the isolated mouse retina in anatomical space.

Tags

Keywords: Mouse RetinaOcular LandmarksDissection ProtocolSpatial OrientationRetinal TopographyDorsalVentralNasalTemporalCorneal BurnRectus MusclesChoroid FissureS-opsin GradientEnucleationRetinal IsolationSclera RemovalIrisLensVitreousRight EyeLeft Eye

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