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RNA原位杂交法定量分析小鼠脑切片中的替代前 mRNA 拼接方法

JoVE Journal
Genética

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JoVE Journal Genética

Quantitative Analysis of Alternative Pre-mRNA Splicing in Mouse Brain Sections Using RNA In Situ Hybridization Assay

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RNA原位杂交法定量分析小鼠脑切片中的替代前 mRNA 拼接方法

DOI:

10.3791/57889-v

•

11:22 min

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August 26, 2018

•

Xuan Guo², Yiqing Zhao³, Hieu Nguyen, Tonghua Liu, Zhenghe Wang³, Hua Lou^3,4

¹Department of Genetics and Genome Sciences,Case Western Reserve University, ²Department of Endocrinology,Dongfang Hospital of Beijing University of Chinese Medicine, ³Case Comprehensive Cancer Center,Case Western Reserve University, ⁴Center for RNA Science and Therapeutics,Case Western Reserve University

Capítulos

00:04Título
01:32Sample Pretreatment
05:00In Situ Hybridization Assay
09:14Results: Use of RNA In Situ Hybridization Assay to Analyze Alternative Pre-mRNA Splicing in Mouse Brain Sections
10:39Conclusion

Summary

Tadução automática

本文介绍了一种利用短反义寡核苷酸检测小鼠脑部替代前 mRNA 拼接模式的原位杂交协议。

Tags

Keywords: Alternative Pre-mRNA Splicing RNA In Situ Hybridization Mouse Brain Sections Quantitative Analysis Isoform-specific Detection Exon Inclusion NF1 Exon 23a Slide Preparation Hybridization Assay

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