Journal
/
Biologia
/
抗菌肽定位的细菌 Spheroplasts 和原生质体的生产与可视化
JoVE Journal
Biologia
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Biologia
Production and Visualization of Bacterial Spheroplasts and Protoplasts to Characterize Antimicrobial Peptide Localization
Please note that all translations are automatically generated. Click here for the English version.

抗菌肽定位的细菌 Spheroplasts 和原生质体的生产与可视化

DOI:
10.3791/57904-v

•

10:13 min

•

August 11, 2018

•

, , , ,
1Biochemistry Program,Wellesley College, 2Department of Chemistry,Wellesley College, 3Department of Biological Sciences,Wellesley College

Capítulos

  • 00:04Título
  • 01:14Gram-negative E. coli Spheroplast Preparation
  • 04:12Gram-positive B. megatherium Protoplast Preparation
  • 05:58<E. coli Spheroplast and B. megatherium Protoplast Visualization and Anticrobial Peptide (AMP) Localization
  • 07:49Results: Representative High-resolution AMP Visualization
  • 09:39Conclusion

Summary

Tadução automática

Tadução automática

在这里, 我们提出了一个生产革兰氏阴性大肠杆菌 (大肠杆菌) spheroplasts 和革兰氏阳性芽孢杆菌巨大(巨大) 原生质体的协议, 以清楚地形象化和快速表征多肽-细菌的相互作用。这为定义膜定位和转运肽提供了系统的方法。

Tags

Bacterial SpheroplastsBacterial ProtoplastsAntimicrobial PeptidesCell Membrane LocalizationGram-negativeGram-positiveE. ColiB. MegateriumCephalexinBacterial FilamentsCell ImagingMembrane-active AgentsCell-penetrating Peptides

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

植物原生质体的荧光激活细胞分选

异源表达在哺乳动物细胞离子通道的突变和功能分析

纳米有限元分析：蛋白质定位的光激活定位显微镜和电子显微镜

有针对性的RNA测序分析表征基因表达和基因组改变

表面拴DNA大分子的可视化与荧光蛋白的DNA结合肽

利用pHluorin标记的受体监视亚细胞定位和贩运

分离块茎原生质体流式细胞仪测量 Endoreduplication 的研究

大豆原生质体分离的简易方法及其在瞬态基因表达分析中的应用

斑马鱼镜头核定位与功能完整性的评价

从植物提取物和分数中识别新型抗菌和抗生物膜分子的系统方法，以防止牙科蛀牙

Read Article