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改良多向分化效力的常规人多能干细胞的化学逆转与幼稚样态
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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Chemical Reversion of Conventional Human Pluripotent Stem Cells to a Naïve-like State with Improved Multilineage Differentiation Potency
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改良多向分化效力的常规人多能干细胞的化学逆转与幼稚样态

DOI:
10.3791/57921-v

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09:07 min

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June 10, 2018

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, , ,
1Department of Oncology, Division of Pediatric Oncology and Institute for Cell Engineering,Johns Hopkins School of Medicine

Capítulos

  • 00:04Título
  • 01:14Bulk Stabilization of Conventional hPSC Cultures for Subsequent Naïve Reversion with a Brief LIF-5i Adaptation
  • 04:38Long-term Maintenance and Expansion of N-hPSC in LIF-3i Medium
  • 07:14Results: Effective Reversion of Human Pluripotent Stem Cells
  • 07:47Conclusion

Summary

Tadução automática

Tadução automática

我们提出一项协议, 以有效, 散装, 快速化学逆转传统的谱系诱导人类多潜能干细胞 (hPSC) 成一个 epigenomically 稳定幼稚的植入啮鼠动物样多潜能状态。该方法在常规 hPSC 线的多向分化上降低了谱系引种基因的表达和显著改善。

Tags

Chemical ReversionConventional Human Pluripotent Stem CellsNaive-like StateMultilineage DifferentiationLIF-3i MethodPre-implantation EpiblastLineage-primed Gene ExpressionEpigenomic StabilityRegenerative MedicineHPSC DifferentiationGene TargetingInterspecies ChimerasLIF-5i MediumCell DissociationFeeder-free Culture

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