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In Vivo Photolabeling of Cells in the Colon to Assess Migratory Potential of Hematopoietic Cells in Neonatal Mice
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JoVE Journal Medicina
In Vivo Photolabeling of Cells in the Colon to Assess Migratory Potential of Hematopoietic Cells in Neonatal Mice

In Vivo Photolabeling of Cells in the Colon to Assess Migratory Potential of Hematopoietic Cells in Neonatal Mice

DOI:
10.3791/57929-v

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08:39 min

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August 10, 2018

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, ,
1Center for Computational and Integrative Biology,Massachusetts General Hospital, 2Mucosal Immunology and Biology Research Center,Massachusetts General Hospital

Capítulos

  • 00:04Título
  • 00:39Colon Cell Photoconversion
  • 03:00Intestinal Lymphocytes Isolation
  • 06:49Results: Migratory Potential of Photolabeled Intestinal Cells
  • 07:56Conclusion

Summary

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The protocol described here utilizes a photolabeling approach in newborn mice to specifically identify immune cells that emigrate from the colon to extra-intestinal sites. This strategy will be useful to study host-microbiome interactions in early life.

Tags

In Vivo PhotolabelingMucosal ImmunologyNeonatal MiceHematopoietic Cell MigrationColon Resident CellsOptogeneticsFiberoptic CannulaUV Light ExposureIntestinal Lymphocytes

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