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Phosphopeptide Enrichment Coupled with Label-free Quantitative Mass Spectrometry to Investigate the Phosphoproteome in Prostate Cancer
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1Graduate Program in Cellular and Molecular Pharmacology, School of Graduate Studies,Rutgers University, The State University of New Jersey, 2Graduate Program in Quantitative Biomedicine, School of Graduate Studies,Rutgers University, The State University of New Jersey, 3Department of Medicine, Division of Medical Oncology,Rutgers Robert Wood Johnson Medical School, 4Crump Institute for Molecular Imaging, Department of Molecular and Medical Pharmacology, Jonsson Comprehensive Cancer Center, UCLA Metabolomics Center, and California NanoSystems Institute,David Geffen School of Medicine, University of California, Los Angeles, 5Mork Family Department of Chemical Engineering and Materials Science,University of Southern California, 6Pharmacology,Rutgers Robert Wood Johnson Medical School, 7Cancer Metabolism and Growth Program,Rutgers Cancer Institute of New Jersey
Capítulos
- 00:04Título
- 00:38Harvesting Tissue
- 01:31Lysate Digestion and Reverse Phase Extraction
- 04:12Immunoprecipitation and Enrichment of pY Peptides and Prewashing of pY Beads
- 06:59Titanium Dioxide Enrichment of pY Peptides and Desalting for MS Analyses
- 09:32Results: Prostate Cancer Phosphopeptide Enrichment and Analysis by Mass Spectrometry
- 11:29Conclusion
