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Amplification of Near Full-length HIV-1 Proviruses for Next-Generation Sequencing
JoVE Journal
Genética
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JoVE Journal Genética
Amplification of Near Full-length HIV-1 Proviruses for Next-Generation Sequencing
DOI:

10:18 min

October 16, 2018

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Capítulos

  • 00:04Título
  • 00:40Lysis of HIV-1-infected Cells and Amplification of Single HIV-1 DNA Proviruses via Nested PCR
  • 04:23DNA Purification and Quantification
  • 08:12Results: Analysis of Amplified Near Full-length HIV-1 Proviruses
  • 09:21Conclusion

Summary

Tadução automática

Full-length individual proviral sequencing (FLIPS) provides an efficient and high-throughput method for the amplification and sequencing of single, near full-length (intact and defective) HIV-1 proviruses and allows for determination of their potential replication-competency. FLIPS overcomes limitations of previous assays designed to sequence the latent HIV-1 reservoir.

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