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Multimodal Bioluminescent and Positronic-emission Tomography/Computational Tomography Imaging of Multiple Myeloma Bone Marrow Xenografts in NOG Mice
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JoVE Journal Pesquisa do Câncer
Multimodal Bioluminescent and Positronic-emission Tomography/Computational Tomography Imaging of Multiple Myeloma Bone Marrow Xenografts in NOG Mice

Multimodal Bioluminescent and Positronic-emission Tomography/Computational Tomography Imaging of Multiple Myeloma Bone Marrow Xenografts in NOG Mice

DOI:
10.3791/58056-v

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05:32 min

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January 07, 2019

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, , , , , , ,
1Greater Los Angeles Veteran Administration Healthcare System, 2San Diego Veterans Administration Healthcare System, 3University of California, Los Angeles, 4Perkin Elmer

Capítulos

  • 00:04Título
  • 00:348226 LUC Engraftment and In Vivo Bioluminescence Monitoring
  • 01:58Targeted Therapy and Tumor Metabolism Monitoring
  • 03:49Results: Representative Multimodal Multiple Myeloma Bone Marrow Xenograft Monitoring
  • 04:53Conclusion

Summary

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Here we use bioluminescent, X-ray, and positron-emission tomography/computed tomography imaging to study how inhibiting mTOR activity impacts bone marrow-engrafted myeloma tumors in a xenograft model. This allows for physiologically relevant, non-invasive, and multimodal analyses of the anti-myeloma effect of therapies targeting bone marrow-engrafted myeloma tumors in vivo.

Tags

Multimodal ImagingBioluminescencePositron Emission TomographyComputational TomographyMultiple MyelomaBone MarrowXenograftsNOG MiceAnti-drug StudiesBiochemical Pathways8226 LuciferaseD-luciferinTemsirolimusFludeoxyglucoseRadiolabeled Probe

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