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Isometric Contractility Measurement of the Mouse Mesenteric Artery Using Wire Myography
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JoVE Journal Medicina
Isometric Contractility Measurement of the Mouse Mesenteric Artery Using Wire Myography

Isometric Contractility Measurement of the Mouse Mesenteric Artery Using Wire Myography

DOI:
10.3791/58064-v

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09:42 min

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August 20, 2018

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, , , , ,
1Model Animal Research Center and MOE Key Laboratory of Model Animal and Disease Study,Nanjing University, 2School of Pharmacy,Nanjing University of Chinese Medicine

Capítulos

  • 00:04Título
  • 00:55Mouse Mesenteric Artery Dissection
  • 02:26Arterial Mounting
  • 05:30Normalization
  • 07:03Artery Contraction Recording
  • 08:18Results: Representative Tracings Showing the Normalization Procedure, Potentiation by High-K+ Solution, and Contraction
  • 08:47Conclusion

Summary

Tadução automática

Tadução automática

The wire myograph technique is used to investigate vascular smooth muscle functions and screen new drugs. We report a detailed protocol for measuring the isometric contractility of the mouse mesenteric artery and for screening new relaxants of vascular smooth muscle.

Tags

Isometric ContractilityWire MyographyMesenteric ArteryVascular Smooth MuscleContractility MeasurementHypertension TherapyHigh PotassiumMulti-channel SystemDrug ScreeningEuthanized MouseGastrointestinal TractMesenteric Artery DissectionHEPES-Tyrode SolutionStereoscopic MicroscopeCalcium-free SolutionArtery SegmentStainless Steel Wire

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