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高通量的高效液氧活化病毒膜融合检测 HIV-1 病毒膜融合抑制剂的研究
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
A High-throughput Cre-Lox Activated Viral Membrane Fusion Assay to Identify Inhibitors of HIV-1 Viral Membrane Fusion
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高通量的高效液氧活化病毒膜融合检测 HIV-1 病毒膜融合抑制剂的研究

DOI:
10.3791/58074-v

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07:22 min

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August 14, 2018

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, , , , ,
1Division of Infectious Diseases, Department of Medicine,Icahn School of Medicine at Mount Sinai, Immunology Institute, 2Department of Biology,New Jersey City University

Capítulos

  • 00:04Título
  • 00:54Preparation of Target Cells for Cell-to-Cell Virus Transmission
  • 02:11Preparation of Donor Cells
  • 03:56Co-culture
  • 05:06Fluorescence-activated Cell Sorting
  • 05:35Results: Identification of a Novel HIV-1 Viral Membrane Fusion Inhibitor
  • 06:49Conclusion

Summary

Tadução automática

Tadução automática

通过流式细胞术或荧光显微镜检测绿色荧光蛋白的表达, 我们描述了以细胞为基础的 HIV-1 融合报告。它可以用来测试病毒进入的抑制剂 (特别是在融合步骤) 在无细胞和细胞对细胞感染系统。

Tags

Cre-LoxViral Membrane FusionHIV-1Drug DiscoveryHigh-throughput ScreeningHIV Fusion InhibitorsBiosafetyJurkat RG Reporter CellsPuromycinCell-to-cell Virus TransmissionGag-iCre DNAElectroporationFicoll

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