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分光光度法测定蓝藻Synechocystis中的蛋白含量

JoVE Journal
Bioquímica
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JoVE Journal Bioquímica

Spectrophotometric Determination of Phycobiliprotein Content in Cyanobacterium Synechocystis

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分光光度法测定蓝藻Synechocystis中的蛋白含量

DOI:

10.3791/58076-v

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08:07 min

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September 11, 2018

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Tomáš Zavřel, Dominik Chmelík², Maria A. Sinetova, Jan Červený

¹Department of Adaptive Biotechnologies, Global Change Research Institute,Czech Academy of Sciences, ²Department of Plant Physiology, Faculty of Science,Masaryk University, ³Laboratory of Intracellular Regulation, Institute of Plant Physiology,Russian Academy of Sciences

Capítulos

00:00Título
01:10Samples Preparation
02:34Cells Homogenization and Pigment Extraction
03:47Phycobiliproteins Quantification
04:47Representative Results
07:30Conclusion

Summary

Tadução automática

在这里, 我们提出了一个协议, 以定量地确定蛋白的内容在蓝藻Synechocystis使用分光光度法。提取过程也成功地应用于其他蓝藻和藻类菌株;然而, 由于颜料吸收谱的变化, 有必要分别测试每种菌株的分光光度方程。

Tags

Keywords: Spectrophotometric Determination Phycobiliproteins Cyanobacterium Synechocystis Phycocyanin Allophycocyanin Pigment-protein Complexes Light-harvesting Antennae Cyanobacteria Productivity Extraction Freeze-drying Homogenization

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