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In Vitro Differentiation of Mouse Granulocyte-macrophage-colony-stimulating Factor (GM-CSF)-producing T Helper (THGM) Cells
JoVE Journal
Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
In Vitro Differentiation of Mouse Granulocyte-macrophage-colony-stimulating Factor (GM-CSF)-producing T Helper (THGM) Cells
DOI:

10:27 min

September 10, 2018

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Capítulos

  • 00:04Título
  • 01:01Murine Splenocyte Preparation
  • 02:10CD4+ T Cell Magnetic Bead Separation
  • 03:48Naïve (CD25-CD44loCD62Lhi) CD4+ T Cell Fluorescence-activated Cell Sorting (FACS) and Granulocyte Macrophage-colony-stimulating Factor (GM-CSF) T Helper (THGM) Cell Differentiation
  • 05:48Mouse THGM Cell Analysis
  • 07:35Results: THGM Cytokine Profile Analysis
  • 09:30Conclusion

Summary

Tadução automática

Here, we present a protocol to differentiate murine granulocyte-macrophage-colony-stimulating-factor-producing T helper (THGM) cells from naive CD4+ T cells, including isolation of naive CD4+ T cells, differentiation of THGM, and analysis of differentiated THGM cells. This method can be applied to studies of the regulation and function of THGM cells.

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