Journal
/
Biologia
/
Characterization of MLKL-mediated Plasma Membrane Rupture in Necroptosis
JoVE Journal
Biologia
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Biologia
Characterization of MLKL-mediated Plasma Membrane Rupture in Necroptosis

Characterization of MLKL-mediated Plasma Membrane Rupture in Necroptosis

DOI:
10.3791/58088-v

•

08:55 min

•

August 07, 2018

•

, , , ,
1Department of Structural Biology,St. Jude Children’s Research Hospital, 2Department of Chemical Biology and Therapeutics,St. Jude Children’s Research Hospital, 3Department of Immunology,St. Jude Children’s Research Hospital

Capítulos

  • 00:04Título
  • 00:50Mixed Lineage Kinase Domain-like (MLKL)-mediated Necroptosis Live-cell Microscopy Imaging
  • 03:26Nuclear Magnetic Resonance (NMR) Spectroscopy MLKL Lipid Binding
  • 05:35Results: Representative MLKL: Plasma Membrane Interaction Analyses
  • 07:26Conclusion

Summary

Tadução automática

Tadução automática

We report methods for characterization of MLKL-mediated plasma membrane rupture in necroptosis including conventional and confocal live-cell microscopy imaging, scanning electron microscopy, and NMR-based lipid binding.

Tags

Keywords: MLKLNecroptosisPlasma Membrane RuptureLive-cell ImagingNuclear Magnetic ResonanceLipid BindingMembrane TranslocationPermeabilizationMouse Embryonic FibroblastsDoxycyclineFK506-binding Protein DimerizerFluorescence MicroscopyCell Death Quantification

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

check_url/pt/58088?article_type=v

Measuring Near Plasma Membrane and Global Intracellular Calcium Dynamics in Astrocytes
check_url/pt/58088?article_type=v

Measuring Plasma Membrane Protein Endocytic Rates by Reversible Biotinylation
check_url/pt/58088?article_type=v

Quantitative Measurement of GLUT4 Translocation to the Plasma Membrane by Flow Cytometry
check_url/pt/58088?article_type=v

Mapping Molecular Diffusion in the Plasma Membrane by Multiple-Target Tracing (MTT)
check_url/pt/58088?article_type=v

Use of a Robot for High-throughput Crystallization of Membrane Proteins in Lipidic Mesophases
check_url/pt/58088?article_type=v

Real-time Analyses of Retinol Transport by the Membrane Receptor of Plasma Retinol Binding Protein
check_url/pt/58088?article_type=v

The Cell-based L-Glutathione Protection Assays to Study Endocytosis and Recycling of Plasma Membrane Proteins
check_url/pt/58088?article_type=v

Imaging Plasma Membrane Deformations With pTIRFM
check_url/pt/58088?article_type=v

Functional Characterization of Na+/H+ Exchangers of Intracellular Compartments Using Proton-killing Selection to Express Them at the Plasma Membrane
check_url/pt/58088?article_type=v

Microperfusion Technique to Investigate Regulation of Microvessel Permeability in Rat Mesentery

Read Article