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用 RNA原位杂交法对形态学组织环境中的遗传变异、短靶点和突变进行可视化
JoVE Journal
Biologia
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JoVE Journal Biologia
Visualizing Genetic Variants, Short Targets, and Point Mutations in the Morphological Tissue Context with an RNA In Situ Hybridization Assay
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用 RNA原位杂交法对形态学组织环境中的遗传变异、短靶点和突变进行可视化

DOI:
10.3791/58097-v

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10:57 min

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August 14, 2018

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, , , , , , , , , ,
1Advanced Cell Diagnostics, Inc

Capítulos

  • 00:04Título
  • 00:41Tissue Preparation
  • 01:30Equipment and Reagent Preparation
  • 03:24Sample Deparaffinization, Dehydration, and Pre-treatment
  • 05:26Probe Hybridization, Signal Amplification, and Detection
  • 07:18Counterstaining, Slide Mounting, and Visualization
  • 08:18Results: Detection of Splice Variants, Short Sequences, and Point Mutation In Situ
  • 10:00Conclusion

Summary

Tadução automática

Tadução automática

在这里, 我们描述了原位杂交检测, 使敏感和特定的检测序列短于50核苷酸的单核苷酸分辨率在单细胞水平。该检测可以手动或自动执行, 可以在组织上下文中实现拼接变体、短序列和突变的可视化。

Tags

Genetic VariantsShort TargetsPoint MutationsRNA In Situ HybridizationCell Type ExpressionSplice VariantsNeurociênciaOncologyExon JunctionsSensitivitySpecificitySingle CellsFormalin FixationParaffin EmbeddingMicrotome SectioningTarget RetrievalWash BufferHematoxylin CounterstainProbe HybridizationAmplification Reagents

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